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trypan blue dye exclusion staining  (Thermo Fisher)
90
Thermo Fisher trypan blue dye exclusion staining
Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by <t>trypan</t> <t>blue</t> <t>exclusion</t> assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test
Trypan Blue Dye Exclusion Staining, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypan+blue+exclusion+dye+staining/pmc06368507-86-10-15
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trypan blue dye exclusion staining - by Bioz Stars, 2026-10
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trypan blue dye exclusion staining  (PanEco Scientific)
90
PanEco Scientific trypan blue dye exclusion staining
Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by <t>trypan</t> <t>blue</t> <t>exclusion</t> assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test
Trypan Blue Dye Exclusion Staining, supplied by PanEco Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypan+blue+exclusion+dye+staining/trypan+blue/pm28681503-95-5-10
Average 90 stars, based on 1 article reviews
trypan blue dye exclusion staining - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
trypan blue exclusion dye assay  (Beyotime)
99
Beyotime trypan blue exclusion dye assay
Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by <t>trypan</t> <t>blue</t> <t>exclusion</t> assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test
Trypan Blue Exclusion Dye Assay, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypan+blue+exclusion+dye+staining/Typan+Blue+Staining+Cell+Viability+Assay+Kit/pm27716619-115-19-32
Average 99 stars, based on 1 article reviews
trypan blue exclusion dye assay - by Bioz Stars, 2026-10
99/100 stars
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trypan blue dye exclusion staining  (Bio-Rad)
96
Bio-Rad trypan blue dye exclusion staining
Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by <t>trypan</t> <t>blue</t> <t>exclusion</t> assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test
Trypan Blue Dye Exclusion Staining, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypan+blue+exclusion+dye+staining/Trypan+Blue/10__3136_slash_fstr__24__627-52-27-37
Average 96 stars, based on 1 article reviews
trypan blue dye exclusion staining - by Bioz Stars, 2026-10
96/100 stars
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trypan blue dye exclusion stain  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc trypan blue dye exclusion stain
Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by <t>trypan</t> <t>blue</t> <t>exclusion</t> assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test
Trypan Blue Dye Exclusion Stain, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypan+blue+exclusion+dye+staining/trypan+blue+dye+exclusion+stain/pmc05901824-75-14-16
Average 90 stars, based on 1 article reviews
trypan blue dye exclusion stain - by Bioz Stars, 2026-10
90/100 stars
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Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by trypan blue exclusion assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

Journal: Cytotechnology

Article Title: Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells

doi: 10.1007/s10616-019-00294-6

Figure Lengend Snippet: Role of PBMCs in AT-MSC death. AT-MSCs were cultivated, for 5 days, alone or in the presence of un-activated or PHA/IL-2-activated PBMCs or activated purified immune cells. a The morphology of AT-MSCs during the co-culture with PBMCs (100× objective). b The cell viability of AT-MSCs measured by trypan blue exclusion assay after the co-culture with PBMCs. c The purity of T-lymphocytes, monocytes and NK cells derived from PBMCs as determined by flow cytometry. d AT-MSCs were cultivated, for 5 days, either alone or in the presence of AT-MSCs after co-culture with PHA/IL-2-activated PBMCs, PHA/IL-2-activated PBMCs lacking CD3, CD14 or CD56 populations, or IL-2 activated purified CD3+, CD14+ or NK cells was measured by trypan blue exclusion assay. Data represent mean percentage ± SEM of viable MSCs from seven independent experiments, each performed in triplicate. *p < 0.05; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

Article Snippet: The number of viable PBMCs was counted by using the Trypan Blue dye exclusion staining (Thermo Fisher Scientific).

Techniques: Purification, Co-Culture Assay, Trypan Blue Exclusion Assay, Derivative Assay, Flow Cytometry

Effects of cytokine activated NK cells on AT-MSCs. AT-MSCs were cultivated, for 5 days, either alone or in the presence of un-activated or IL-2-, IL-12-, IL-15- or IL-21-activated NK cells. a Morphology of AT-MSCs (100× objective). b AT-MSC viability measured by trypan blue exclusion assay. c AT-MSC mortality as determined by 7 amino-actinomycin (7-AAD) staining assay. d Lactate dehydrogenase (LDH) assay for measuring cellular toxicity. The percent of cytotoxicity was calculated as described in “Materials and methods” section. Data represent mean percentage ± SEM of seven independent experiments, each performed in triplicate. **p < 0.01; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

Journal: Cytotechnology

Article Title: Reciprocal immuno-biological alterations occur during the co-culture of natural killer cells and adipose tissue-derived mesenchymal stromal cells

doi: 10.1007/s10616-019-00294-6

Figure Lengend Snippet: Effects of cytokine activated NK cells on AT-MSCs. AT-MSCs were cultivated, for 5 days, either alone or in the presence of un-activated or IL-2-, IL-12-, IL-15- or IL-21-activated NK cells. a Morphology of AT-MSCs (100× objective). b AT-MSC viability measured by trypan blue exclusion assay. c AT-MSC mortality as determined by 7 amino-actinomycin (7-AAD) staining assay. d Lactate dehydrogenase (LDH) assay for measuring cellular toxicity. The percent of cytotoxicity was calculated as described in “Materials and methods” section. Data represent mean percentage ± SEM of seven independent experiments, each performed in triplicate. **p < 0.01; ***p < 0.001 versus MSCs; paired one way ANOVA followed by Bonferroni post-test

Article Snippet: The number of viable PBMCs was counted by using the Trypan Blue dye exclusion staining (Thermo Fisher Scientific).

Techniques: Trypan Blue Exclusion Assay, Staining, Lactate Dehydrogenase Assay